Modeling the efficiency of filovirus entry into cells in vitro: Effects of SNP mutations in the receptor molecule.

Modeling the efficiency of filovirus entry into cells in vitro: Effects of SNP mutations in the receptor molecule.

Blog Article

Interaction between filovirus glycoprotein (GP) and the Niemann-Pick C1 (NPC1) protein is essential for membrane fusion during virus entry.Some single-nucleotide polymorphism (SNPs) in two surface-exposed loops of NPC1 are known to reduce viral infectivity.However, the dependence of differences in entry efficiency on SNPs remains unclear.Using vesicular stomatitis virus pseudotyped with Ebola and weboost coax cable Marburg virus GPs, we investigated the cell-to-cell spread of viruses in cultured cells expressing NPC1 or SNP derivatives.

Eclipse and virus-producing phases were assessed by sebenza 31 insingo magnacut in vitro infection experiments, and we developed a mathematical model describing spatial-temporal virus spread.This mathematical model fit the plaque radius data well from day 2 to day 6.Based on the estimated parameters, we found that SNPs causing the P424A and D508N substitutions in NPC1 most effectively reduced the entry efficiency of Ebola and Marburg viruses, respectively.Our novel approach could be broadly applied to other virus plaque assays.